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Updated: Jul 12, 2026
05:59
Trypsinizing and Subculturing Mammalian Cells
Published on: June 12, 2008
Mammalian cell culture: technology and physiology
Advances in Experimental Medicine and Biology
|January 1, 1984
Summary
Optimizing mammalian cell culture involves understanding physical and chemical parameters. Simple temperature and oxygen adjustments can significantly increase cell density and improve product quality in large-scale bioreactors.
Area of Science:
- Biotechnology
- Biochemical Engineering
- Cell Culture Technology
Background:
- Mammalian cell culture has seen significant advancements in large-scale microcarrier and suspension methods.
- Serum costs and product quality necessitate a thorough examination of physical and chemical parameters in these systems.
Purpose of the Study:
- To investigate methods for enhancing cell density and product quality in large-scale mammalian cell culture.
- To analyze the impact of physical transport phenomena and chemical parameters on cell growth.
Main Methods:
- Utilized temperature measurements and oxygen potential adjustments in 100-liter and 12-liter batch reactors, respectively.
- Interpreted results using theoretical relationships of Navier-Stokes, Fourier, and Fick for transport phenomena.
- Analyzed spent cell growth media chemistry across 3-, 12-, and 100-liter reactors using the Monod growth curve.
Main Results:
- Achieved more than double the final harvest cell density in 100-liter reactors via temperature control.
- Increased cell density in 12-liter reactors by augmenting the liquid's oxygen potential.
- Monod kinetics effectively explained cell growth based on analyzed media chemistries.
Conclusions:
- Optimizing mammalian cell culture requires integrating principles of chemistry, thermodynamics, and transport phenomena.
- Significant cost savings and improved product quality are achievable through a multidisciplinary approach to cell culture technology and physiology.
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