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Calcium sensitivity is modified by contraction.

A M Gordon, E B Ridgway, D A Martyn

    Advances in Experimental Medicine and Biology
    |January 1, 1984
    PubMed
    Summary
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    Muscle contraction influences calcium sensitivity and binding through crossbridge interactions, demonstrating true hysteresis. This phenomenon, observed in various muscle fibers, indicates that the force-calcium relationship is history-dependent.

    Area of Science:

    • Muscle Physiology
    • Biophysics
    • Calcium Signaling

    Background:

    • Crossbridge interactions are fundamental to muscle contraction.
    • Calcium ions (Ca) regulate muscle force generation.
    • The precise mechanisms by which contraction history affects Ca sensitivity are not fully understood.

    Purpose of the Study:

    • To investigate whether crossbridge interaction modifies calcium sensitivity and binding.
    • To determine if the observed effects represent true hysteresis.
    • To explore the impact of muscle length changes on calcium binding.

    Main Methods:

    • Injected barnacle muscle fibers with aequorin to monitor intracellular Ca levels.
    • Utilized voltage clamp and controlled length conditions in muscle fiber preparations.

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  • Measured force in "skinned" muscle fibers exposed to varying Ca concentrations and solutions.

    • Main Results:

    • Muscle contraction history alters Ca sensitivity, leading to hysteresis in the force-pCa relationship.
    • Briefly elevating force early in contraction resulted in higher steady-state force at the same Ca level.
    • Muscle shortening or stretching during contraction affected sarcoplasmic Ca levels, suggesting altered Ca binding.

    Conclusions:

    • Crossbridge interactions significantly modify Ca sensitivity and binding during muscle contraction.
    • The observed force-pCa relationship exhibits true hysteresis, not just slow equilibrium.
    • Muscle length changes can influence Ca binding, likely at myofilament sites.