[Isolation of monospecific antiserum to rat IgE]

Immunochemically pure IgE was obtained from the serum of LOW rats carrying IgE-plasmocytome by the immunochemical methods (ion-exchange chromatography and gel filtration). This IgE served as an antigen for immunization of a rabbit to produce a specific antiserum. The monospecificity of this antiserum was achieved by its sorption with normal serum proteins.