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Macrotubules induced by halothane: in vitro assembly

R E Hinkley

    Journal of Cell Science
    |August 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Volatile anesthetic halothane forms macrotubules from microtubule protofilaments. This process is concentration-dependent, calcium-stimulated, and distinct from typical microtubule assembly.

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    Area of Science:

    • Cell Biology
    • Biochemistry
    • Neuroscience

    Background:

    • Microtubules are essential cytoskeletal components involved in various cellular processes.
    • Volatile anesthetics are known to affect neuronal function, but their precise molecular mechanisms are not fully understood.

    Purpose of the Study:

    • To investigate the in vitro formation of macrotubules induced by the volatile anesthetic halothane.
    • To elucidate the structural assembly pathway of halothane-induced macrotubules.

    Main Methods:

    • Utilized microtubule-enriched fractions from crayfish nerve cords for in vitro studies.
    • Employed sequential microscopic analysis to observe macrotubule assembly.
    • Investigated the effects of halothane concentration, calcium, and glycerol on macrotubule formation.

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    Main Results:

    • Macrotubules assemble from helical ribbons of 18-20 microtubule protofilaments, forming structures ~48 nm in diameter.
    • Macrotubule assembly rate is dependent on halothane concentration and stimulated by calcium.
    • Glycerol pretreatment inhibited halothane-induced macrotubule formation and induced rapid reassembly into typical microtubules.

    Conclusions:

    • Microtubule and macrotubule assembly require distinct conditions.
    • Halothane-induced macrotubule formation likely results from a direct interaction between halothane molecules and microtubule subunits.
    • These findings provide insights into the molecular mechanisms of volatile anesthetic action on cytoskeletal dynamics.