Proteolytic digestion studies of chromatin core-histone structure. Identification of limit peptides from histone H2B

Trypsin digestion of chicken erythrocyte chromatin results in a well-defined set of limit peptides derived from the core histones (P1-P5). Two peptides running in sodium dodecyl sulphate gel electrophoresis at the positions of P2 and P3 have been identified as histone H2B residues 21-125 and 24-125. No C-terminal residues are therefore lost from histone H2B. The N-terminal sequence removed correlates well with that having low sequence conservation and not with that showing the greatest basicity. The same correlation was reported previously by us for histone H2A.