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[Characteristics of experimental antibiotic-induced dysbacteriosis]
Abstract:
Changes in the microflora of the large and small intestines in mice and guinea pigs after the oral administration of canamycin (a hardly absorbable antibiotic) and ampiox (an easily absorbable antibiotic) in different doses. The administration of these antibiotics in different doses (therapeutic, subtherapeutic and over therapeutic) led to an increase in the number of opportunistic microorganisms and the contamination of the small intestine by these organisms. These changes were also well pronounced in guinea pigs, normally having no enterobacteria. After the administration of the antibiotics was stopped, opportunistic microorganisms were gradually eliminated from the small intestine. The rate of decontamination depended on the administered dose of the antibiotic: the higher the dose was the longer the process of the decontamination of the small intestine lasted. An increase in the amount of opportunistic microbes in the large intestine and the decontamination of the small intestine occurred simultaneously with the decrease in the amount of lactobacilli and bifidobacteria in both the small and large intestines.
Insights
Oral antibiotics like canamycin and ampiox disrupt gut microflora, increasing opportunistic microbes in the small intestine. Higher antibiotic doses prolonged the recovery of gut bacteria, affecting beneficial lactobacilli and bifidobacteria.
Area of Science:
- Microbiology
- Pharmacology
- Gastroenterology
Background:
- Antibiotic administration can significantly alter the composition of the gut microbiota.
- Understanding the impact of different antibiotic absorption rates on microflora is crucial for clinical practice.
Purpose of the Study:
- To investigate the effects of canamycin (poorly absorbed) and ampiox (readily absorbed) on intestinal microflora in mice and guinea pigs.
- To evaluate the dose-dependent impact of these antibiotics on opportunistic microorganisms and beneficial bacteria.
Main Methods:
- Oral administration of canamycin and ampiox at therapeutic, subtherapeutic, and supra-therapeutic doses to mice and guinea pigs.
- Analysis of changes in the microflora of the large and small intestines.
- Monitoring the recovery of intestinal microflora after antibiotic cessation.
Main Results:
- Antibiotic treatment led to an increase and small intestine contamination by opportunistic microorganisms.
- These effects were more pronounced in guinea pigs, which normally lack enterobacteria.
- The dose of antibiotic administered correlated with the duration of opportunistic microbe elimination from the small intestine.
- A decrease in lactobacilli and bifidobacteria was observed concurrently with an increase in opportunistic microbes in the large intestine.
Conclusions:
- Oral antibiotics disrupt the intestinal microflora balance, promoting opportunistic pathogen growth.
- The absorption rate and dosage of antibiotics influence the extent and duration of microflora disruption and recovery.
- Antibiotic therapy necessitates careful consideration of potential long-term effects on gut microbial ecosystems.