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Updated: Aug 9, 2026

An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes
Published on: November 21, 2016
Effect of ionophores on lymphocyte cellular metabolism
Abstract:
The effect of valinomycin, nigericin, gramicidin S and D, A23187 and X537A on respiration and cellular ATP content of rat spleen lymphocytes is presented. It has been found that while valinomycin and nigericin interfere with mitochondrial functions gramicidin D does not show an appreciable effect. These results are explained in terms of different ability of ionophores to re-distribute among intracellular membranes. A23187 and X537A, added with Ca2+, strongly enhanced O2 consumption and reduced cellular ATP content.
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