[Method of quantitative determination of androgens and their metabolites]

Simple and rapid procedure is developed for quantitative estimation of 3H-androgen metabolites, which is carried out without extraction of labelled steroids from tissue homogenates after incubation with a substrate. After incubation the samples were liophylized and the labelled metabolites were separated using thin-layer chromatography on Silufol plates. The zones, corresponding to labelled metabolites, were scratched out with silica gelsheet and radioactivity was estimated. The procedure excluded practically a loss of labelled steroids in the samples. The procedure was used for estimation of 3H-androgen metabolites in vitro--3H-testosterone, 3H1 dihydrotestosterone and 3H-5 alpha-androsterone-3 beta, 17 beta-diol in homogenates of hypophyses and hypothalamuses from rat males (intact and castrated) and in adenoma of human prostatic gland.