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Calcium-sequestering vesicles and contractile flagellar roots

J L Salisbury

    Journal of Cell Science
    |December 1, 1982
    PubMed
    Summary
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    Calcium sequestration in vesicles drives flagellar root contraction in Tetraselmis subcordiformis. Calcium deposits appear in the cytosol upon stimulation, revealing a mechanism for regulating intracellular calcium levels.

    Area of Science:

    • * Cell biology
    • * Marine biology
    • * Phycology

    Background:

    • * The green alga Tetraselmis subcordiformis possesses striated flagellar roots involved in motility.
    • * Understanding the role of intracellular calcium in the contraction of these structures is crucial for elucidating cellular mechanics.

    Purpose of the Study:

    • * To investigate the intracellular localization of calcium during the contraction of Tetraselmis subcordiformis flagellar roots.
    • * To identify calcium-sequestering organelles and their role in regulating intracellular calcium levels.

    Main Methods:

    • * Employed calcium pyroantimonate and calcium oxalate cytochemistry.
    • * Utilized energy-dispersive X-ray microanalysis for elemental detection.
    • * Applied 'calcium shock' stimulation to induce flagellar root contraction.

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    Main Results:

    • * Identified anterior vesicles (0.25–0.50 µm) as calcium-sequestering organelles.
    • * Observed periodic calcium antimonate (Ca-Sb) deposits in contractile flagellar roots (CFRs) upon stimulation.
    • * Found Ca-Sb deposits throughout the cytosol during CFR contraction, absent in extended states.

    Conclusions:

    • * Vesicles play a key role in sequestering calcium for flagellar root contraction.
    • * Cytosolic calcium dynamics are implicated in the mechanism of CFR contraction.
    • * Findings contribute to understanding intracellular calcium regulation in motile algae.