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Modular electronics for flow cytometry and sorting: the LACEL system

R D Hiebert, J H Jett, G C Salzman

|March 1, 1981

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The newly developed LACEL system offers fast, flexible data acquisition and processing for flow cytometry. Its modular design supports multiparameter measurements and advanced sorting capabilities.

Area of Science:

Biotechnology
Instrumentation
Data Science

Background:

Flow cytometry generates complex multiparameter data.
Existing systems may lack flexibility or speed for advanced applications.

Purpose of the Study:

Introduce LACEL, a novel data acquisition and processing system for flow cytometry.
Highlight its modularity, speed, and advanced features for multiparameter analysis and cell sorting.

Main Methods:

Developed a modular electronic system for data acquisition.
Implemented 16-bit data transfer via standard I/O interfaces.
Integrated 8-fold coincidence logic for timing flexibility in multiparameter measurements.

Main Results:

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The LACEL system processes up to eight analog parameters.

It supports 16-bit data transfer for high-resolution measurements.

Features 8-fold coincidence logic adaptable to noncoincidental timing.

Enables defining sorting criteria based on four parameters for two directions.

Allows simultaneous on-line processing of two experiments.

Conclusions:

LACEL provides a fast, flexible, and powerful solution for flow cytometric data acquisition and processing.
Its advanced features, including coincidence logic and sorting capabilities, enhance multiparameter analysis.
The system's modularity ensures adaptability to diverse research needs in flow cytometry.