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[Effect on freezing on hydroxylation in isolated hepatocytes]
Voprosy Meditsinskoi Khimii
|May 1, 1981
Summary
Rapid freezing impairs liver cell (hepatocyte) functions. Ethylene glycol (EG) and dimethyl sulfoxide (DMSO) cryoprotectants partially preserve microsomal oxidation and mitochondrial activity during freezing.
Area of Science:
- Cell Biology
- Cryobiology
- Biochemistry
Context:
- Hepatocytes are crucial for metabolic functions.
- Cryopreservation is vital for preserving biological samples.
- Understanding cryoprotectant effects on cellular functions is essential.
Purpose:
- To investigate the impact of rapid freezing on hepatocyte functions.
- To evaluate the efficacy of ethylene glycol (EG) and dimethyl sulfoxide (DMSO) in mitigating cryoinjury.
- To assess the preservation of microsomal oxidation and mitochondrial activity post-freezing.
Summary:
- Rapid freezing to -196°C significantly impairs microsomal oxidation and uncouples mitochondrial respiration and oxidative phosphorylation in hepatocytes.
- Multistep freezing with ethylene glycol (EG) preserved 70.3% of microsomal oxidation activity.
- Multistep freezing with dimethyl sulfoxide (DMSO) preserved 78% of microsomal oxidation activity.
- Mitochondrial function was maintained at 30% with EG and 40% with DMSO after freezing.
Impact:
- Cryoprotectants like EG and DMSO offer partial protection to cellular functions during freezing.
- Findings inform strategies for optimizing cryopreservation protocols for hepatocytes.
- Highlights the differential effects of cryoprotectants on specific cellular components.