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[Method for obtaining Paramphistomum daubneyi, Dinnik, 1962 metacercariae]
Veterinarno-Meditsinski Nauki
|January 1, 1981
Summary
This study details the life cycle of Paramphistomum daubneyi, focusing on its development in Galba truncatula snails. Optimal conditions for cercariae shedding and metacercariae production were identified for research purposes.
Area of Science:
- Veterinary Parasitology
- Malacology
- Life Cycle Research
Context:
- Paramphistomum daubneyi is an economically significant trematode parasite affecting livestock.
- Understanding its life cycle is crucial for developing effective control strategies.
- Galba truncatula snails serve as the intermediate host for P. daubneyi.
Purpose:
- To elucidate the developmental stages of P. daubneyi within G. truncatula.
- To determine optimal environmental conditions for cercariae shedding and metacercariae formation.
- To establish a reliable method for obtaining infective metacercariae for experimental use.
Summary:
- Miracidia of P. daubneyi infected G. truncatula snails, with cercariae maturing in 32-50 days.
- Cercariae shedding was enhanced at 15-20°C on Plantago lanceolata leaves, forming metacercariae.
- Temperatures above 25°C inhibited cercariae elimination; up to 1628 metacercariae were recovered per snail.
Impact:
- Provides critical data on the environmental factors influencing P. daubneyi transmission.
- Establishes a reproducible protocol for snail intermediate host infection and metacercariae harvesting.
- Facilitates further research into P. daubneyi pathogenesis and control measures in animal health.