[Comparative study of methods for isolating glucose isomerase from Streptomyces albogriseolus mycelium]

Different procedures for disintegrating the biomass of Streptomyces albogriseolus were compared, and the recovery of glucose isomerase was analysed for each of the procedures. The following techniques were tested: disintegration of the mycelium with ultrasound and with pressure, autolysis in the presence of lysozyme and in its absence. The yields of the enzyme and total protein were found to depend on the time during which the biomass was treated. The effect of different procedures for isolating the enzyme on its recovery and activity is discussed.