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Cell adhesion on model substrata: threshold effects and receptor modulation

J D Aplin, R C Hughes

    Journal of Cell Science
    |August 1, 1981
    PubMed
    Summary
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    Cell adhesion and spreading on lectin-coated surfaces depend on lectin concentration and cell surface carbohydrate expression. Higher lectin matrix density is needed compared to fibronectin for cell morphological transformation.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Materials Science

    Background:

    • Cell adhesion is crucial for biological processes.
    • Lectins are proteins that bind specific carbohydrates on cell surfaces.
    • Fibronectin is a known extracellular matrix protein involved in cell adhesion.

    Purpose of the Study:

    • To investigate cell adhesion and morphological changes on lectin-derivatized surfaces.
    • To quantify the matrix density of lectins required for cell spreading.
    • To compare lectin-mediated adhesion with fibronectin-mediated adhesion.

    Main Methods:

    • Immobilizing various lectins onto glass surfaces.
    • Culturing trypsinized BHK cells on these derivatized surfaces.
    • Observing and quantifying cell attachment and morphology.

    Related Experiment Videos

  • Testing cells with varying resistance to ricin.

    • Main Results:

    • BHK cells attach and spread on lectin-coated glass above a threshold lectin concentration.
    • Higher lectin matrix densities (10-40 fold) are required for spreading compared to fibronectin.
    • Cells with reduced ricin-binding carbohydrates need higher ricin matrix densities for spreading.
    • All cell lines spread equally on concanavalin A matrices.

    Conclusions:

    • Demonstrates quantitative interactions between cell surface molecules and matrix components for adhesion.
    • Proposes a model for cell spreading on protein-coated substrata.
    • Highlights the role of lectin concentration and cell surface carbohydrate availability in cell adhesion and morphology.