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Related Experiment Videos

Transfer factor: a murine model

M E Williams, C A Kauffman

    Infection and Immunity
    |January 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed a subprimate model for transfer factor studies using BALB/c mice. A low-molecular-weight substance from spleen cells transferred purified protein derivative (PPD) sensitivity, confirmed by in vivo and in vitro assays.

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    Area of Science:

    • Immunology
    • Cellular immunology

    Background:

    • Transfer factor research is extensive in humans.
    • A reliable subprimate model for transfer factor studies is lacking.
    • Understanding transfer factor mechanisms requires appropriate animal models.

    Purpose of the Study:

    • To establish a subprimate model for studying transfer factor.
    • To investigate the transfer of cellular sensitivity using mouse spleen cell extracts.
    • To characterize the substance responsible for transferring PPD sensitivity.

    Main Methods:

    • BALB/c mice were immunized with complete Freund adjuvant.
    • A low-molecular-weight substance was extracted from disrupted spleen cells.
    • Recipient mice received the spleen cell extract.

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  • Sensitivity transfer was assessed via PPD footpad swelling (in vivo) and splenic lymphocyte transformation (in vitro).

    • Main Results:

    • A low-molecular-weight substance transferred PPD sensitivity to recipient mice.
    • Transfer of sensitivity was confirmed by both in vivo and in vitro assays.
    • An inverse correlation was observed between PPD and concanavalin A responses in recipient splenic lymphocytes.
    • Control extracts from saline-treated mice did not transfer PPD sensitivity.

    Conclusions:

    • A BALB/c mouse model effectively demonstrates transfer factor-mediated PPD sensitivity.
    • Low-molecular-weight spleen cell components are implicated in this transfer.
    • This model provides a basis for further investigation into transfer factor mechanisms in a subprimate system.