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[Ultrastructure of the initial stages of interaction between halprowiae (Chlamydiae) and host cells]
Abstract:
The initial stages of the interaction of halprowiae (strain CP-1), isolated in Reiter's syndrome, with a monolayer culture of cells L-929 were studied. The electron-microscopic examination of the cells every 2 hours during the first 24 hours after the inoculation allowed to follow the processes of adsorption, endocytosis of elementary bodies and their transformation into reticulate bodies by the "decondensation" of nucleoid, which indicated the beginning of the vegetative (reproductive) stage of the developmental cycle. During all the periods of observation halprowiae were enclosed into the membranes of phagocytic vacuoles formed by host cells. Besides, in other vacuoles (probably, phagolysosomes) halprowiae at various stages of destruction could be observed. 8--10 hours after inoculation the phagosomes containing reticulate bodies began to merge, thus forming an inoculation the phagosomes containing reticulate bodies began to merge, thus forming an intracytoplasmic inclusion. In this inclusion a new generation of the agent was formed by binary-like fission. At the beginning of the vegetative stage halprowiae seem to be highly sensitive to the action of exo- and endogenic factors which may cause their L-transformation. Depending on the reaction of the host cell at the initial stages of interaction with halprowiae, 3 ways of such interaction are seemingly possible: developmental cycle, destruction in phagolysosomes. L-transformation.
Insights
Halprowiae, isolated from Reiter's syndrome, were studied interacting with L-929 cells. Electron microscopy revealed their intracellular developmental cycle, including transformation, replication, and potential L-transformation.
Area of Science:
- Microbiology
- Cell Biology
- Pathogen-Host Interactions
Background:
- Reiter's syndrome is associated with specific microbial agents.
- Understanding pathogen-host cell interactions is crucial for infectious disease research.
Purpose of the Study:
- To investigate the initial interaction stages between halprowiae (strain CP-1) and L-929 cells.
- To elucidate the intracellular developmental cycle of halprowiae within host cells.
Main Methods:
- Electron microscopy was used to observe halprowiae-L-929 cell interactions over 24 hours.
- Cell cultures were inoculated with halprowiae (strain CP-1).
Main Results:
- Halprowiae were observed undergoing adsorption and endocytosis, transforming into reticulate bodies within phagocytic vacuoles.
- Intracytoplasmic inclusions formed via phagosome fusion, leading to new halprowiae generation through binary-like fission.
- Evidence suggests halprowiae sensitivity to host factors, potentially leading to L-transformation.
Conclusions:
- The study details the intracellular life cycle of halprowiae within L-929 cells, including replication and potential L-transformation.
- Three interaction pathways (developmental cycle, destruction, L-transformation) are proposed based on host cell response.