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Inhibitory effects of 2'-deoxy-L-thymidine 5'-triphosphate on human immunodeficiency virus reverse transcriptase and
T Yamaguchi1, M Saneyoshi, K Shudo
1Department of Biological Sciences, Nishi-Tokyo University, Yamanashi, Japan.
Nucleic Acids Symposium Series
|January 1, 1993
Abstract:
Inhibitory effects of 2'-deoxy-L-thymidine 5'-triphosphate (L-dTTP) which is an enantiomer of the natural substrate (D-dTTP) on the activity of mammalian DNA polymerases, Escherichia coli DNA polymerase I and HIV-1 reverse transcriptase were examined. Interestingly, L-dTTP showed remarkable inhibitory effect on HIV-1 reverse transcriptase in competitive fashion with respect to the substrate dTTP. In contrast, eukaryotic cell nuclear DNA polymerases alpha and beta were not or slightly inhibited by L-dTTP.