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Related Experiment Videos

Correlation between phenotypic characteristics and DNA relatedness within Enterococcus faecium strains

L M Teixeira1, R R Facklam, A G Steigerwalt

  • 1Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.

Journal of Clinical Microbiology
|June 1, 1995
PubMed
Summary

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Atypical Enterococcus faecium strains with variable sugar fermentation and vancomycin resistance were confirmed as E. faecium variants. DNA-DNA reassociation and protein profiling support expanding the phenotypic description of E. faecium.

Area of Science:

  • Microbiology
  • Bacterial Taxonomy
  • Clinical Microbiology

Background:

  • Enterococcal strains from clinical specimens exhibit variable phenotypic characteristics.
  • Some atypical strains resemble Enterococcus faecium but show unusual acid production and vancomycin resistance.
  • Distinguishing between variants and new species is crucial for accurate identification and treatment.

Purpose of the Study:

  • To determine if atypical enterococcal strains with variable phenotypes are variants of Enterococcus faecium or distinct species.
  • To clarify the phenotypic spectrum of Enterococcus faecium.
  • To assess the utility of molecular methods in differentiating these strains.

Main Methods:

  • Phenotypic characterization including acid production from glycerol, mannitol, raffinose, and sorbitol, and vancomycin susceptibility testing.

Related Experiment Videos

  • Whole-cell protein profiling using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
  • DNA-DNA reassociation experiments for species identification.

    • Main Results:

    • Atypical strains, including mannitol-negative variants, showed high DNA-DNA relatedness (≥70% at optimal, ≥60% under stringent conditions) to the E. faecium type strain.
    • Protein profiles of atypical strains were similar to typical E. faecium and distinct from Enterococcus faecalis.
    • Enterococcus faecalis strains exhibited low DNA-DNA relatedness to the E. faecium type strain (12-16%).

    Conclusions:

    • Atypical enterococcal strains with variable fermentation patterns and vancomycin resistance are confirmed as variants of Enterococcus faecium.
    • The phenotypic description of Enterococcus faecium should encompass these observed variations.
    • Molecular methods like DNA-DNA hybridization and protein profiling are reliable for accurate enterococcal species identification.