Neuronal mapping studies on the central nervous system of the pulmonate snail Helisoma trivolvis

The need for a generalized system for mapping neurons in the central ring ganglia of the freshwater pulmonate snail, Helisoma trivolvis, has recently become apparent. In the present study we have used retrograde staining of nerves to identify neurons projecting to the periphery. This map was combined with histochemical information to produce a map of neuronal clusters and landmark cells. Our mapping strategy was formulated to make direct comparison with maps already established for closely related species, i.e., Lymnaea stagnalis.