Related Experiment Videos
Selectivity in electrophoretically mediated microanalysis by control of product detection time
B J Harmon1, I Leesong, F E Regnier
1Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-1393.
Analytical Chemistry
|November 1, 1994
Summary
Electrophoretically mediated microanalysis (EMMA) allows precise control over reaction product detection times by leveraging differences in electrophoretic mobility. This technique enables selective analysis of analytes and substrates, even in complex mixtures.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Electrophoretically mediated microanalysis (EMMA) utilizes differential electrophoretic mobility for selective detection.
- Controlling reaction product detection time is crucial for accurate chemical analysis.
Purpose of the Study:
- To describe a method for manipulating reaction product detection times in EMMA.
- To demonstrate the selective determination of enzymes and substrates.
- To enable simultaneous EMMA determinations of multiple analytes.
Main Methods:
- Exploiting differential electrophoretic mobility between analytes and reaction products.
- Controlling the timing of the analytical reaction to maneuver product detection windows.
- Separating reaction products from non-reacting matrix interferants.
Main Results:
- Demonstrated selective control over reaction product detection times.
- Achieved independent manipulation of product profiles from interferants.
- Successfully determined both enzymes and substrates using the EMMA technique.
- Enabled simultaneous determination of multiple enzymes or substrates.
Conclusions:
- EMMA offers a unique capability to selectively control product detection times.
- This control allows for interference-free determination of analytes.
- The technique facilitates simultaneous multi-analyte determinations, enhancing analytical efficiency.