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[Comparative study on minimum inhibitory concentration of gonococcal strains according to their auxogroups and
Abstract:
Auxotypes, serovars and minimum inhibitory concentration (MIC) of various antibiotics are investigated on 85 gonococcal strains isolated from the patient with gonorrhea from 1984 to 1986 in Japanese Red Cross Medical Center. As to the auxotypes 33 strains were identified to be Proto, 33 to be Pro, 10 to be Arg and 9 to be PAU. No AHU strain was identified. Serovars were divided into 1A and 1B. MIC of 22 kinds of antibiotics including penicillins, cephems, spectinomycin, tetracycline and new quinolones were determined. Cumulative MIC curves were found to be shifted to the right according to the order of auxotypes Arg, PAU, Proto, Pro in the most antibiotics. Serovar 1A was found to have lower MIC as compared to 1B, though only 5 strain of 1A were in the study. Seventy-eight % of isolates were Proto or Pro, so-called Asian auxotype which were revealed to have higher MIC not only of penicillin but also of the other antibiotics. Nine strains were identified as PAU which was lately identified in Canada. This might suggest that gonococcal strains are spreading over the world in spite of the local predominance of auxotype distribution.
Insights
This study analyzed 85 Neisseria gonorrhoeae strains, finding that common Asian auxotypes (Proto, Pro) showed higher antibiotic resistance. This suggests global spread of gonorrhea strains with varying resistance patterns.
Area of Science:
- Microbiology and Infectious Diseases
- Antimicrobial Resistance
- Epidemiology
Background:
- Neisseria gonorrhoeae causes gonorrhea, a significant public health concern.
- Understanding gonococcal strain characteristics, including auxotypes and serovars, is crucial for tracking antimicrobial resistance.
- Previous studies have indicated geographical variations in gonococcal auxotype distribution.
Purpose of the Study:
- To investigate the auxotypes, serovars, and antibiotic susceptibility of gonococcal strains.
- To determine the minimum inhibitory concentrations (MICs) of various antibiotics against these strains.
- To analyze the relationship between auxotype/serovar and antibiotic resistance patterns in gonorrhea.
Main Methods:
- Isolation and characterization of 85 Neisseria gonorrhoeae strains from patients between 1984-1986.
- Determination of auxotypes (Proto, Pro, Arg, PAU) and serovars (1A, 1B).
- Antimicrobial susceptibility testing for 22 antibiotics, including penicillins, cephems, spectinomycin, tetracycline, and new quinolones, measuring MICs.
Main Results:
- The majority of strains were identified as Proto (33) or Pro (33), characteristic of the 'Asian auxotype'.
- Higher minimum inhibitory concentrations (MICs) were observed for Proto and Pro auxotypes compared to Arg and PAU auxotypes across most antibiotics.
- Serovar 1A strains showed lower MICs than Serovar 1B, although with a small sample size for 1A.
Conclusions:
- The 'Asian auxotype' (Proto/Pro) strains exhibit increased resistance to a range of antibiotics.
- The identification of PAU strains, previously noted in Canada, suggests potential global dissemination of specific gonococcal strains.
- Findings highlight the importance of monitoring auxotype distribution and antimicrobial susceptibility for effective gonorrhea control.