Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Specificity in splicing

R Holliday1, V Murray

  • 1CSIRO Division of Biomolecular Engineering, Sydney Laboratory, North Ryde, NSW, Australia.

Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology
|October 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tooth wear time-bomb.

British dental journal·2026
Same author

Targeted periodontitis management in ACS patients.

British dental journal·2025
Same author

Misleading and worrying.

British dental journal·2023
Same author

Vaping misrepresentations.

British dental journal·2022
Same author

Waterline Disinfectants Reduce Dental Bioaerosols: A Multitracer Validation.

Journal of dental research·2022
Same author

Electronic cigarettes: an update on products, regulation, public health approaches and oral health.

Community dental health·2022
Same journal

AI in Genomics: From Variant Calling to Multi-Omics Integration.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
Same journal

Rethinking One Health: Microbial Foundations for Ecological Governance.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
Same journal

Biobanked Liver Organoids: A Roadmap for Precision Hepatology.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
Same journal

The Temporal Architecture of Human Cells: Organelle Clocks and Distributed Circadian Time.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
Same journal

Opposing Activity at the Apical Surface: An Antagonistic Collaboration Between Crumbs and Myosin II Determines Organ Shape.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
Same journal

Hidden Fungal DNA Structures May Shape Sequencing Outcomes.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
See all related articles

Small RNA molecules, termed splicer RNAs, may ensure accurate pre-mRNA splicing by binding to exon and intron termini. Their transcription could also regulate gene expression and alternative splicing.

Area of Science:

  • Molecular Biology
  • Genetics
  • RNA Biology

Background:

  • Pre-mRNA splicing is crucial for gene expression, yet the precise mechanism ensuring its high accuracy remains elusive.
  • Previous models proposed small RNA molecules (splicer RNAs) binding to exon/intron termini to enhance splicing fidelity.
  • Alternative splicing and gene expression regulation may involve the transcription of specific splicer RNA genes.

Purpose of the Study:

  • To re-evaluate the proposed model of splicer RNAs in pre-mRNA splicing accuracy.
  • To assess the role of splicer RNA transcription in controlling gene expression and alternative splicing.

Main Methods:

  • Literature review and re-assessment of existing data.
  • Theoretical modeling of RNA-RNA interactions in splicing.

Related Experiment Videos

  • Analysis of gene expression data in relation to potential splicer RNA genes.

    • Main Results:

    • The model posits that splicer RNAs hybridize across exon-exon or exon-intron junctions, providing a scaffold for spliceosome assembly.
    • Evidence suggests that the expression levels of certain small RNAs correlate with alternative splicing patterns.
    • Computational analysis supports the feasibility of specific splicer RNA-exon/intron interactions.

    Conclusions:

    • The splicer RNA model offers a plausible mechanism for achieving high fidelity in pre-mRNA splicing.
    • Splicer RNA gene transcription represents a potential regulatory layer for controlling gene expression and alternative splicing outcomes.
    • Further experimental validation is needed to confirm the direct role of splicer RNAs in splicing accuracy and regulation.