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Dentin permeability to bacterial proteins in vitro
1Department of Restorative Dentistry and Endodontology, University of Connecticut Health Center, School of Dental Medicine, Farmington.
Journal of Endodontics
|March 1, 1994
Summary
Bacterial proteins from Porphyromonas gingivalis can pass through dentin, altering its hydraulic conductance. This study investigated protein filtration and binding within dentin, revealing mechanical interactions.
Area of Science:
- Microbiology
- Biomaterials Science
- Dental Research
Background:
- Bacterial component passage through dentin is a growing research area.
- Previous studies demonstrated diffusive transport of Porphyromonas gingivalis proteins via in vitro models.
Purpose of the Study:
- To investigate the filtration of P. gingivalis proteins through dentin.
- To quantify concentration changes post-filtration.
- To analyze the physical basis of protein-dentin binding.
Main Methods:
- Determined hydraulic conductance (Lp) of 10 dentin specimens.
- Used phosphate-buffered saline and P. gingivalis ATCC 33277 suspension (200 µg/ml protein) in sequential experiments.
- Measured Lp changes and protein retention.
Main Results:
- Observed significant, non-permanent alterations in dentin's Lp.
- Demonstrated variable retention of bacterial proteins across samples.
- Found a positive correlation between bacterial protein retention and Lp reduction.
Conclusions:
- Dentin microstructural changes likely cause Lp reduction.
- Bacterial protein retention occurs on dentin surfaces or within tubules.
- Intratubular interactions appear mechanical rather than chemical.