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Effect of esculentoside H on release of tumor necrosis factor from mouse peritoneal macrophages
1Research Laboratory of Natural and Synthetic Drugs, College of Pharmacy, Second Military Medical University, Shanghai, China.
Abstract:
Effect of esculentoside H (EH) on release of tumor necrosis factor (TNF) from murine peritoneal macrophage (Mphi) in vitro was studied. The results showed that EH (12.5-200 micrograms.ml-1) induced the thioglycolate-broth elicited peritoneal Mphi to release TNF into supernatants in a dose-dependent manner, and higher levels of TNF activity were detected in the supernatants from EH-stimulated calcimycin-primed Mø culture. EH-induced TNF release had a different type of kinetics compared with that of lipopolysaccharides (LPS). LPS-induced release of TNF increased rapidly until 6 h after LPS stimulation, then declined gradually, while EH-induced TNF release increased gradually after EH stimulation and reached its peak at approximately 24 h later. These results suggested that the anti-tumor mechanisms of Phytolaccaceae may be related to the capacity of EH for TNF release.
Insights
Esculentoside H (EH) stimulates macrophages to release tumor necrosis factor (TNF), a key immune signaling molecule. This finding suggests EH may contribute to anti-tumor effects by modulating immune responses.
Area of Science:
- Immunology
- Pharmacology
- Natural Products Chemistry
Background:
- Tumor necrosis factor (TNF) plays a critical role in immune responses and inflammation.
- Phytolaccaceae family plants are known for their medicinal properties, but their anti-tumor mechanisms require further elucidation.
Purpose of the Study:
- To investigate the effect of esculentoside H (EH) on tumor necrosis factor (TNF) release from murine peritoneal macrophages (Mphi) in vitro.
- To compare the kinetics of EH-induced TNF release with that of lipopolysaccharides (LPS).
Main Methods:
- Murine peritoneal macrophages were cultured and stimulated with varying concentrations of esculentoside H (EH).
- TNF activity in the cell culture supernatants was measured.
- Kinetic analysis of TNF release was performed and compared to LPS-stimulated controls.
Main Results:
- Esculentoside H (EH) induced a dose-dependent release of TNF from Mphi.
- EH-stimulated TNF release showed distinct kinetics, peaking around 24 hours, differing from the rapid, transient release induced by LPS.
- Priming Mphi with calcimycin enhanced EH-induced TNF release.
Conclusions:
- Esculentoside H (EH) effectively induces TNF release from macrophages.
- The distinct kinetics of EH-induced TNF release suggest a unique immunomodulatory mechanism.
- These findings support the hypothesis that EH's anti-tumor properties may be linked to its ability to induce TNF release.