[A method of calculating complexed calcium in patients with in-vitro simulated hypo- and hypercalcemia]

Assessment of plasma protein calcium-binding capacity in disease involves measurements of Ca fractions in native plasma and its samples after in vitro stimulation of hypo- and hypercalcemia; such measurements include multiple and labor-consuming anaerobic ultrafiltration procedures. Values of Ca bound to plasma complexons and effective constants of their associations with Ca were experimentally assessed in 20 normal subjects, 7 patients before and 20 ones after kidney transplantation with the use of ultrafiltration. A method for estimation of integrated calcium in a sample lot has been developed, based on a single experimental value of integrated calcium in native plasma and the mean association constant in health. Experimental and estimated values of liganded Ca were in good correlation, this confirming the rightfulness of clinical use of the suggested method. This method essentially simplifies assessment of plasma proteins Ca-binding properties and requires lesser blood volumes.