[Fluorescence spectroscopy in selective percutaneous nucleotomy using the excimer laser--experimental studies]

Area of Science:

• Biomedical Optics
• Laser-Tissue Interaction
• Spectroscopy

Context:

• Minimally invasive surgical techniques require precise tissue differentiation.
• Percutaneous laser nucleotomy aims to selectively ablate disc material.
• Current methods may lack the specificity for differentiating target tissues.

Purpose:

• To evaluate fluorescence spectroscopy for selective laser ablation.
• To determine if spectral analysis can differentiate between nucleus pulposus, anulus fibrosus, and surrounding vertebral tissues.
• To assess the feasibility of fluorescence spectroscopy for guiding percutaneous laser nucleotomy.

Summary:

• A XeCl excimer laser was used to excite fluorescence in human and pig vertebral segments.
• Distinct fluorescence signatures were observed for anulus fibrosus compared to other tissues.
• Intensity ratios at specific wavelengths (350, 385, 435 nm) enabled clear tissue discrimination.
• Fluorescence properties were independent of excitation energy and surrounding medium.

Impact:

• Demonstrates the potential of fluorescence spectroscopy for real-time tissue discrimination during laser procedures.
• Suggests a viable method for enhancing the selectivity of percutaneous laser nucleotomy.
• Paves the way for improved precision and safety in spinal interventions.