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Updated: Jul 27, 2026
07:23
Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
Published on: June 15, 2016
Control of TGEV mRNA transcription
J Hiscox1, D H Pocock, P Britton
1Division of Molecular Biology, A.F.R.C., Institute for Animal Health, Newbury, Berkshire, United Kingdom.
Advances in Experimental Medicine and Biology
|January 1, 1993
Summary
This study investigates how coronavirus mRNAs are made using leader primed transcription. Researchers developed a method to measure mRNA levels, linking leader sequence length to transcriptional control.
Area of Science:
- Molecular Biology
- Virology
- Genetics
Background:
- Coronavirus proteins are produced from subgenomic mRNAs with shared 3' termini and unique 5' extensions.
- The generation of these mRNAs is thought to involve a leader primed transcription mechanism.
Purpose of the Study:
- To investigate the role of leader binding site length in the transcriptional control of individual coronavirus mRNAs.
- To develop and utilize a virus-specific mRNA isolation method for quantifying mRNA synthesis.
Main Methods:
- Development of a virus-specific mRNA isolation technique.
- Infection of LLC-PK1 cells with Transmissible Gastroenteritis Virus (TGEV).
- Measurement of relative mRNA quantities synthesized during infection.
Main Results:
- The study established a method to measure the relative abundance of synthesized mRNAs during TGEV infection.
- The relative quantities of individual mRNAs can be determined and correlated with variations in leader binding site size.
Conclusions:
- The findings suggest a correlation between leader binding site length and the transcriptional control of coronavirus mRNA production.
- This research provides insights into the regulation of viral gene expression in coronaviruses.
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