Related Experiment Videos
Molecular design of artificial hydrolytic nucleases and ribonucleases
M Komiyama1, T Inokawa, T Shiiba
1Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Japan.
Nucleic Acids Symposium Series
|January 1, 1993
Abstract:
Artificial nucleases and ribonucleases, which selectively hydrolyze the specific phosphodiester linkages in DNAs and RNAs, are prepared by the attachment of organic and inorganic catalysts to DNA oligomers as sequence-recognizing moieties. A hybrid of ethylenediamine and a 19-mer DNA selectively hydrolyzes tRNA(Phe) at the 3'-side of C63. Site-selective hydrolysis of DNAs has been accomplished by the lanthanide complexes bound to DNA oligomers. No concurrent oxidative cleavage of the ribose or the deoxyribose takes place.