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Related Experiment Videos

[Solid phase techniques in blood group serology]

H Uthemann1, L Sturmfels, V Lenhard

  • 1BIOTEST AG, Dreieich, BRD.

Infusionstherapie Und Transfusionsmedizin
|June 1, 1993
PubMed
Summary

Solid-phase red blood cell adherence assays in microplates offer advantages over hemagglutination. These automated methods improve blood group typing, Rh phenotyping, and antibody screening for better transfusion safety.

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Area of Science:

  • Immunology
  • Hematology
  • Clinical Pathology

Context:

  • Hemagglutination assays are traditional methods for blood typing and antibody screening.
  • Solid-phase red blood cell adherence assays offer enhanced sensitivity and automation potential.
  • Microplate adaptation of these assays facilitates high-throughput processing and objective data analysis.

Purpose:

  • To describe the adaptation and application of solid-phase red blood cell adherence assays in microplates.
  • To detail the use of these assays for ABO grouping, Rh typing, Rh phenotyping, antibody screening and identification, and crossmatching.
  • To highlight the advantages of microplate-based assays over conventional hemagglutination techniques.

Summary:

  • Solid-phase red blood cell adherence assays utilizing microplates are presented as superior alternatives to hemagglutination.
  • These assays enable semi-automated processing, spectrophotometric reading, and computer-based interpretation.
  • The paper outlines the application of these advanced techniques across various blood group serology tests, including typing, phenotyping, antibody analysis, and crossmatching.

Impact:

  • Facilitates more efficient and accurate blood group serology testing.
  • Enhances transfusion safety through improved antibody screening and crossmatching.
  • Promotes the adoption of automated, microplate-based diagnostic platforms in clinical laboratories.

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