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Updated: Jul 28, 2026

Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria
Published on: September 7, 2012
Increased extracellular calcium concentrations in the isolated rat heart: effects on mitochondrial contact site
A Bakker1, M De Bie, I Bernaert
1University of Antwerp (UIA), Dept. of Medicine, Belgium.
Abstract:
In our previous study, mitochondrial creatine kinase (Mi-CK) activity is localised cytochemically in heart tissue. The activity was found to be exclusively localised in mitochondrial contact sites and the surface density of Mi-CK was increased upon myocardial stimulation (Biermans et al., 1989). As calcium is involved as an intracellular messenger of stimulation, we compared hearts noradrenaline-stimulated in vivo with isolated hearts perfused with buffers containing different calcium concentrations on the surface density Mi-CK.
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