Related Experiment Videos
Labelled polycyanoacrylate nanoparticles for human in vivo use
G E Ghanem1, C Joubran, R Arnould
1Department of Nuclear Medicine, Institut Jules Bordet, Université Libre de Bruxelles, Belgium.
Summary
Researchers developed gamma-emitting isotope labeling for cyanoacrylate nanoparticles, enabling organ imaging in humans. This advancement allows for better tracking of drug delivery systems, particularly for anti-cancer drugs.
Area of Science:
- Nanotechnology
- Radiochemistry
- Pharmacology
Background:
- Isobutyl and isohexyl cyanoacrylate nanoparticles serve as drug carriers, especially for anti-cancer agents.
- Previous pharmacokinetic and biodistribution studies relied on beta-emitters, limiting in-vivo imaging in humans.
- Scintigraphy requires nanoparticle labeling with gamma-emitting isotopes for human organ distribution and uptake studies.
Purpose of the Study:
- To develop methods for labeling cyanoacrylate nanoparticles with gamma-emitting isotopes for human imaging.
- To assess the stability and efficiency of these novel radiolabeled nanoparticles.
Main Methods:
- Developed labeling techniques using gamma-emitters like radioactive iodine, indium (111In), and technetium (99mTc).
- Utilized diethylenetriaminepentaacetic acid (DTPA) as a spacer for chelating indium and technetium.
- Achieved high labeling yields (>80%) and assessed stability in human plasma.
Main Results:
- Successful labeling of nanoparticles with gamma-emitting isotopes was achieved.
- Radiolabeled nanoparticles (111In and 99mTc) demonstrated stability in human plasma.
- Administration to rabbits and humans showed 60-75% accumulation in the reticulo-endothelial system.
Conclusions:
- Developed effective methods for gamma-emitting isotope labeling of cyanoacrylate nanoparticles.
- These radiolabeled nanoparticles are suitable for human scintigraphy, enabling organ distribution and uptake studies.
- The DTPA-chelation method allows for potential use with other gamma-emitting cations.