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Expression immunoassay. Antigen quantitation using antibodies labeled with enzyme-coding DNA fragments

T K Christopoulos1, N H Chiu

  • 1Department of Chemistry and Biochemistry, University of Windsor, Ontario, Canada.

Analytical Chemistry
|December 1, 1995
PubMed
Summary

This study introduces a novel expression immunoassay using DNA fragments that code for enzymes. This method achieves high sensitivity for detecting antigens by measuring enzymatic activity after DNA expression and amplification.

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