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MT-MMP expression and localisation in human lung and breast cancers
M Polette1, B Nawrocki, C Gilles
1I.N.S.E.R.M. U.314, C.H.R. Maison Blanche, Reims, France.
Virchows Archiv : an International Journal of Pathology
|April 1, 1996
Summary
The study found that membrane-type matrix metalloproteinase (MT-MMP) is highly expressed in lung and breast cancers, suggesting its role in tumor progression and invasion. This enzyme may facilitate cancer cell invasion through basement membrane degradation.
Area of Science:
- Oncology
- Biochemistry
- Molecular Biology
Background:
- Matrix metalloproteinases (MMPs) play a role in extracellular matrix degradation.
- MT-MMP is a recently described MMP capable of activating gelatinase A, which degrades basement membranes.
Purpose of the Study:
- To investigate the expression and potential role of MT-MMP in primary pulmonary squamous cell carcinomas and breast carcinomas.
- To analyze the relationship between MT-MMP expression and tumor progression in these cancers.
Main Methods:
- In situ hybridization to detect MT-MMP mRNA.
- Northern blot analysis for MT-MMP and gelatinase A transcripts.
- Immunohistochemistry to localize MT-MMP protein expression.
Main Results:
- MT-MMP transcripts were found in both tumor and stromal cells in lung squamous cell carcinomas.
- In breast carcinomas and lung adenocarcinomas, MT-MMP mRNA was mainly in stromal cells near tumor clusters.
- MT-MMP and gelatinase A transcripts showed parallel expression in lung and breast cancers.
- Immunohistochemistry revealed extensive MT-MMP distribution in tumor and stromal cells of both cancer types.
Conclusions:
- High expression of MT-MMP in lung and breast cancers suggests a significant role in tumor progression.
- Cooperation between cancer cells and stromal cells in MT-MMP expression may contribute to tumor invasion.