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A low-copy number plasmid mediating beta-lactamase production by Xanthomonas maltophilia
1Cardeza Foundation for Hematological Research, Thomas Jefferson University, Philadelphia, PA, USA.
Abstract:
To delineate the mechanisms contributing to the high level of antimicrobial resistance often demonstrated by Xanthomonas maltophilia, plasmid DNA was isolated from 5 clinical isolates and analyzed. Purified plasmid DNA from a single isolate contained a 6.5 kb plasmid (pXM222) and a 5.6 kb plasmid, (pTHB). Transformation of pTHB into E. coli HB101 resulted in the expression of resistance to all penicillins tested and cefazolin.
Insights
This study investigated antimicrobial resistance in Xanthomonas maltophilia by analyzing plasmid DNA. A specific plasmid, pTHB, was found to confer resistance to penicillins and cefazolin when transferred to E. coli.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Xanthomonas maltophilia frequently exhibits high levels of antimicrobial resistance.
- Understanding the genetic basis of this resistance is crucial for effective treatment strategies.
Purpose of the Study:
- To identify and characterize plasmids responsible for antimicrobial resistance in clinical isolates of Xanthomonas maltophilia.
- To determine the specific resistance mechanisms conferred by these plasmids.
Main Methods:
- Plasmid DNA was isolated from five clinical isolates of Xanthomonas maltophilia.
- Plasmid DNA was analyzed, and specific plasmids were purified.
- Plasmid transformation into E. coli HB101 was performed to assess gene expression.
Main Results:
- Two plasmids, pXM222 (6.5 kb) and pTHB (5.6 kb), were identified in a single isolate.
- Transformation of the pTHB plasmid into E. coli HB101 resulted in the expression of resistance.
- The pTHB plasmid conferred resistance to all tested penicillins and cefazolin.
Conclusions:
- The pTHB plasmid plays a significant role in conferring penicillin and cefazolin resistance in Xanthomonas maltophilia.
- Plasmid-mediated resistance mechanisms are a key factor in the antimicrobial resistance of Xanthomonas maltophilia.