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Cryopreservation of equine embryos
1Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, USA.
The Veterinary Clinics of North America. Equine Practice
|April 1, 1996
Abstract:
Principles and procedures for cryopreservation of equine embryos are described. Embryos less than 250 microM in diameter can be cryopreserved successfully if glycerol is used as the cryoprotectant. Cooling is takes place in such a way that most of the water leaves the cells before intracellular ice forms, and glycerol is removed after thawing without undue osmotic swelling of cells. Vitrification procedures also show promise for small embryos. Satisfactory procedures for cryopreserving embryos of more than 250 microM in diameter are not yet available.