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Serum lamotrigine analysis by capillary electrophoresis
1Department of Pathology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157, USA.
Journal of Chromatography. B, Biomedical Applications
|August 9, 1996
Summary
Capillary zone electrophoresis offers a rapid and accurate method for analyzing lamotrigine, an antiepileptic drug, in patient serum. This technique provides a quick alternative to HPLC, with results correlating well and aiding in therapeutic drug monitoring.
Area of Science:
- Analytical Chemistry
- Pharmacology
- Clinical Chemistry
Background:
- Lamotrigine is a widely used antiepileptic medication.
- Accurate therapeutic drug monitoring is crucial for optimizing lamotrigine efficacy and safety.
- Existing analytical methods may require further optimization for speed and efficiency.
Purpose of the Study:
- To develop and validate a capillary zone electrophoresis (CE) method for the quantitative analysis of lamotrigine in human serum.
- To compare the performance of the CE method with a high-performance liquid chromatography (HPLC) method.
- To assess the potential for CE in routine therapeutic drug monitoring of lamotrigine.
Main Methods:
- Serum samples were deproteinized using acetonitrile and acidified with acetic acid.
- Capillary zone electrophoresis was performed with rapid migration of lamotrigine.
- Analyte quantification was achieved using an internal standard and linearity was assessed.
- Method validation included comparison with an established HPLC technique.
Main Results:
- The CE method demonstrated linearity between 0.5-10 mg/l with a rapid analysis time of approximately 5 minutes.
- Lamotrigine migrated quickly, separating effectively from interfering substances.
- CE results showed a strong correlation with HPLC method values (r = 0.97).
- Mean serum lamotrigine concentration in patients was 3.7 mg/l, with a 24% increase observed after beta-glucuronidase incubation, suggesting the presence of conjugated forms.
Conclusions:
- Capillary zone electrophoresis is a suitable, rapid, and reliable technique for lamotrigine analysis in serum.
- The CE method offers a viable alternative to HPLC for therapeutic drug monitoring of lamotrigine.
- Further investigation into the deconjugation of lamotrigine in serum may enhance analytical accuracy.