Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein secondary structural types are differentially coded on messenger RNA

T A Thanaraj1, P Argos

  • 1European Molecular Biology Laboratory, Heidelberg, Germany. thanaraj@ebi.ac.uk

Protein Science : a Publication of the Protein Society
|October 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prediction of protein folding pathways: Bovine pancreatic trypsin inhibitor.

Cytotechnology·2012
Same author

Function of alternative splicing.

Gene·2005
Same author

ASD: the Alternative Splicing Database.

Nucleic acids research·2003
Same author

Conservation of human alternative splice events in mouse.

Nucleic acids research·2003
Same author

Prediction and statistical analysis of alternatively spliced exons.

Progress in molecular and subcellular biology·2002
Same author

Categorization and characterization of transcript-confirmed constitutively and alternatively spliced introns and exons from human.

Human molecular genetics·2002
Same journal

Macromolecular crowding inhibits degradation of alpha-synuclein amyloid fibrils induced by cathepsins and MMP9.

Protein science : a publication of the Protein Society·2026
Same journal

Sequence-encoded differences in the conformational ensembles of CITED transcriptional activation domains impact coactivator binding.

Protein science : a publication of the Protein Society·2026
Same journal

The phospholipid biosynthesis enzyme PlsB contains three distinct domains for membrane association, lysophosphatidic acid synthesis, and dimerization.

Protein science : a publication of the Protein Society·2026
Same journal

Structural basis of ligand selectivity in FAD/NAD(P)H-dependent dehydrogenases: insights from trypanothione reductase and type II NADH dehydrogenase.

Protein science : a publication of the Protein Society·2026
Same journal

Achieving protease substrate-specific inhibition by mAb dual functional selections.

Protein science : a publication of the Protein Society·2026
Same journal

How important are quantum mechanical effects in controlling biological functions: Enzymes, electron transfer and bird navigation.

Protein science : a publication of the Protein Society·2026
See all related articles

Messenger RNA (mRNA) codon usage influences protein structure. Fast-translating mRNA regions preferentially encode alpha helices, while slow regions encode beta strands and coils in Escherichia coli.

Area of Science:

  • Molecular Biology
  • Biophysics
  • Genetics

Background:

  • Codon usage bias in highly expressed genes suggests selection for translational efficiency.
  • The relationship between mRNA translation speed and the resulting protein's tertiary structure is not fully understood.

Purpose of the Study:

  • To investigate the correlation between mRNA codon translation speed and the secondary structure of encoded proteins in Escherichia coli.
  • To explore the influence of codon usage on protein folding and tertiary structure formation.

Main Methods:

  • Analyzing codon frequencies in highly expressed Escherichia coli genes to estimate translation speed.
  • Classifying mRNA regions based on summed codon frequencies (tricodons).
  • Correlating mRNA translation speed with amino acid residue conformation in protein tertiary structures.

Related Experiment Videos

Main Results:

  • A significant correlation was observed between mRNA translation speed and protein secondary structure.
  • Translationally fast mRNA regions preferentially encode alpha helices.
  • Translationally slow mRNA regions tend to encode beta strands and coil regions.

Conclusions:

  • Codon usage in mRNA is linked to the topological features of the encoded proteins.
  • Ribosome mechanics and peptide exit tunnel structure support the observed relationship between translation speed and protein conformation.
  • This finding provides insights into the interplay between gene expression, protein folding kinetics, and protein structure.