Determination of fludarabine phosphate in human plasma using reversed phase high-performance liquid chromatography

A rapid, simple and accurate HPLC method for the determination of fludarabine phosphate in human plasma is presented. Fludarabine phosphate from plasma was successfully purified. The samples were chromatographed on a LiChrosorb RP-18 (10 microns) column after purification using a Bakerbond Spe column. The mobile phase was methanol/phosphoric buffer pH 4.15 (20:80). The detection was carried out at 262 nm. The method was tested for linearity (range from 100 to 900 ng/ml), recovery (ca. 99.8%) and precision (C.V. = 3.2%).