Related Experiment Video
Updated: Aug 4, 2026

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
Published on: January 16, 2016
Kinetics of hydrolysis of ceftazidime in aqueous solutions
1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, School of Medicine, Poznań, Poland.
Abstract:
The kinetics of hydrolysis of ceftazidime in aqueous solutions at 323 K, 333 K, 343 K and 353 K over the pH-range (0.43-9.76) and at 295 K, 303 K and 308 K over the pH-range (9.17-13.16) has been investigated. The decomposition was followed by HPLC and UV spectral methods. The pH-rate profile was accounted for by the specific acid catalyzed reactions and also by assuming spontaneous water-catalyzed decomposition of molecule ceftazidime in various grade of dissociation. Various buffer substances were found to exhibit general acid and base catalysis of the degradation. Thermodynamic parameters of the reaction: energy, entropy and enthalpy of activation and the frequency factor for the specific rate constants were determined.
More Related Videos
05:46Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation
Published on: August 1, 2018
10:21Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins
Published on: June 20, 2019
Related Concept Videos
Weak Acid Solutions
Factors Affecting Solubility
Acid Halides to Carboxylic Acids: Hydrolysis
As shown below, the mechanism involves a nucleophilic attack by water at the carbonyl carbon to form a tetrahedral intermediate. This is followed by the reformation of the carbon–oxygen π bond along with the departure of a halide ion. A final proton transfer step yields carboxylic acid...
Esters to Carboxylic Acids: Acid-Catalyzed Hydrolysis
During hydrolysis, the ester is first activated towards nucleophilic attack through the protonation of the carboxyl oxygen atom by the acid catalyst. The protonation makes the ester carbonyl carbon more electrophilic. In the next step, water acts as a nucleophile and adds to the...
Factors Influencing Drug Absorption: Drug Dissolution
Phase I Reactions: Hydrolytic Reactions
An important hydrolytic reaction is ester hydrolysis. Ester bonds, often found in prodrugs, are broken down, increasing the solubility of drugs like aspirin and lidocaine for more straightforward elimination. Amide hydrolysis is another critical reaction, targeting amide bonds prevalent...