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Engineering thermolysin-like proteases whose stability is largely independent of calcium
O R Veltman1, G Vriend, B van den Burg
1Department of Genetics, University of Groningen, Haren, The Netherlands.
FEBS Letters
|March 24, 1997
Abstract:
Thermal stability of the thermolysin-like protease produced by Bacillus stearothermophilus (TLP-ste) is highly dependent on calcium at concentrations in the millimolar range. We describe the rational design and production of a fully active TLP-ste variant whose stability is only slightly dependent on calcium concentration.