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Epoxy-embedded versus nonembedded TEM examination of the resin-dentin interface
B Van Meerbeek1, J D Eick, S J Robinson
1BIOMAT-Department of Operative Dentistry and Dental Materials, School of Dentistry, Oral Pathology and Maxillo-Facial Surgery, Catholic University of Leuven, Belgium. BART.VANMEERBEEK@MED.KULEUVEN.AC.BE
Journal of Biomedical Materials Research
|May 1, 1997
Summary
Transmission electron microscopy (TEM) can create artifactual hybrid layers. This study found no ultrastructural differences in hybrid layers formed by two adhesive systems, regardless of epoxy embedding, suggesting reliable resin infiltration.
Area of Science:
- Dental materials science
- Biomaterials engineering
- Microscopy techniques
Background:
- Transmission electron microscopy (TEM) is crucial for evaluating resin-dentin interfaces.
- Epoxy resin embedding can create artifactual hybrid layers, potentially masking incomplete resin infiltration.
- Accurate assessment of the hybrid layer is vital for understanding adhesive performance.
Purpose of the Study:
- To investigate the impact of epoxy embedding on TEM analysis of the resin-dentin interface.
- To compare the ultramorphology of hybrid layers formed by Optibond and Scotchbond Multi-Purpose under different preparation methods.
- To determine if epoxy embedding introduces artifacts that obscure incomplete resin infiltration.
Main Methods:
- Resin-dentin interfaces were created using Optibond and Scotchbond Multi-Purpose adhesive systems.
- Specimens were prepared using both standard epoxy embedding and non-embedding (HMDS immersion) techniques for TEM.
- Ultrastructural analysis of the hybrid layer was performed using TEM on nondemineralized sections.
Main Results:
- Both Optibond and Scotchbond Multi-Purpose formed hybrid layers irrespective of the preparation method.
- No significant ultrastructural differences in hybrid layer formation were observed between epoxy-embedded and non-embedded sections.
- No evidence of microporosities or gaps was found at the base of the hybrid layer, indicating effective resin infiltration.
Conclusions:
- Epoxy resin embedding does not appear to create artifactual hybrid layers that obscure resin infiltration in the evaluated systems.
- The preparation method (epoxy embedding vs. non-embedding) did not affect the observed ultrastructure of the hybrid layer.
- Both evaluated adhesive systems demonstrated effective resin infiltration of the demineralized collagen scaffold.