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Membrane anchored synthetic peptides as a tool for structure-function analysis of pertussis toxin and its target
L von Olleschik-Elbheim1, A el Bayâ, M A Schmidt
1Institut für Infektiologie, Universität Münster, Germany.
Advances in Experimental Medicine and Biology
|January 1, 1997
Abstract:
Solid phase fixed peptides are useful tools for the investigation of enzyme substrate interactions. We could show that interactions of target sequences, like e.g. the acceptor domain of G-proteins for the of mono ADP-ribosylation by pertussis toxin and the toxin itself can be studied by this method. Reaction specificity is identical to modification of free peptides. The method is extremely versatile and has enormous potential if the focus is on the effects of substrate modification or substrate length.