Liver cysteine proteinases in macrophage depression induced by gadolinium chloride

Liver lysosomal enzymes during macrophage depression (gadolinium chloride, 7 mg/kg, intravenously) and macrophage stimulation (zymosan, 100 mg/kg, intravenously) have been studied. It was shown that gadolinium chloride treatment of rats reduced the rate of carbon particles phagocytosis at 24 and 48 h after the single administration. Decreased endocytic capacity of Kupffer cells was confirmed also by electron microscopy. Gadolinium chloride induced labilization of liver lysosomes (increased free activity of cathepsins B and L); there was no changes of specific activity of liver cysteine proteinases (24 h). Gadolinium chloride prevented death of rats after administration of non-sonicated particular zymosan particles, resulting to 70% survival, compare with the 17% survival in group with zymosan alone. We can summarize that macrophages depression by gadolinium chloride abolish symptoms of inflammation in zymosan-model, influencing on cysteine proteinases of Kupffer cells.