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Related Experiment Videos

Choroid plexus ion transporter expression and cerebrospinal fluid secretion

R F Keep1, J Xiang, L J Ulanski

  • 1Department of Surgery (Neurosurgery), University of Michigan, Ann Arbor, USA.

Acta Neurochirurgica. Supplement
|January 1, 1997
PubMed
Summary

Changes in blood pH and potassium levels affect choroid plexus transporters involved in cerebrospinal fluid (CSF) homeostasis. These alterations in ion transporter expression suggest compensatory mechanisms that may influence CSF production.

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Area of Science:

  • Neuroscience
  • Physiology
  • Molecular Biology

Background:

  • The choroid plexus plays a crucial role in cerebrospinal fluid (CSF) secretion and homeostasis.
  • Choroid plexus transporters, including the Cl-/HCO3- exchanger (AE2 isoform) and Na+/K(+)-ATPase, are implicated in both CSF secretion and ion balance.

Purpose of the Study:

  • To investigate the control mechanisms and regulatory parameters influencing the expression of AE2 and Na+/K(+)-ATPase in the choroid plexus.
  • To determine the role of blood pH and osmolality in regulating AE2 mRNA levels.
  • To examine the effect of plasma potassium levels on Na+/K(+)-ATPase subunit protein expression.

Main Methods:

  • Northern blot analysis was used to quantify AE2 mRNA levels in rat choroid plexus following induced alkalosis or changes in blood osmolality.

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  • Western blot analysis was employed to assess the protein levels of Na+/K(+)-ATPase alpha 1 and beta 1 subunits in response to variations in plasma potassium concentration.

    • Main Results:

    • Six hours of alkalosis significantly increased AE2 mRNA levels by 40% in the choroid plexus, indicating a role in CSF pH homeostasis.
    • Alterations in blood osmolality did not significantly affect AE2 mRNA expression.
    • A positive correlation was observed between plasma potassium concentration and the protein levels of both alpha 1 and beta 1 subunits of Na+/K(+)-ATPase, suggesting a role in CSF potassium homeostasis.

    Conclusions:

    • Choroid plexus ion transporter expression, specifically AE2 and Na+/K(+)-ATPase, is modulated by changes in blood pH and plasma potassium levels.
    • Despite these modulations, CSF production rates were not significantly altered, implying the existence of compensatory mechanisms.
    • Understanding these regulatory and compensatory mechanisms could offer insights for therapeutic interventions aimed at controlling CSF production.