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Preliminary study on hydrophobic interaction chromatography of Chromobacterium viscosum lipase on polypropylene
M M Diogo1, J M Cabral, J A Queiroz
1Departamento de Química, Universidade da Beira Interior, Covilhã, Portugal.
Journal of Chromatography. A
|March 26, 1998
Abstract:
The purification of Chromobacterium viscosum lipase was performed using a polypropylene glycol-Sepharose gel. The influence of the mobile phase composition on the chromatographic behaviour of Chromobacterium viscosum lipase was studied and it was found that the retention of lipase depends on the salt used and increased with ionic strength. Using 20% (w/v) ammonium sulphate in the eluent, a total retention of lipase on the column was obtained.