Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Screening aspartyl proteases with combinatorial libraries

C D Carroll1, M Orlowski

  • 1Pharmacopeia, Inc., Princeton, New Jersey 08540, USA.

Advances in Experimental Medicine and Biology
|April 30, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From bench to bedside: initial experience with the Primus drug-coated balloon catheter.

Minerva cardioangiologica·2012
Same author

Comparison of air and immersion chilling on meat quality and shelf life of marinated broiler breast fillets.

Poultry science·2008
Same author

Marination of turkey breast fillets to control the growth of Listeria monocytogenes and improve meat quality in deli loaves.

Poultry science·2006
Same author

Evaluation of acid gastric secretion with 24-hour pH-metry in patients with acute and chronic hepatitis induced by HBV.

Medical science monitor : international medical journal of experimental and clinical research·2002
Same author

Correlation between liver damage and degree of gastric mucose colonization by Helicobacter pylori in subjects with parenchymatous liver damage.

Medical science monitor : international medical journal of experimental and clinical research·2002
Same author

[Staphylococcus aureus sepsis--still life threatening disease].

Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego·2002
Same journal

Mammalian Respiratory Chain Complex Assemblies and Their Links to Mitochondria Stress-Induced Human Diseases.

Advances in experimental medicine and biology·2026
Same journal

Enzyme Assemblies in Nucleotide Metabolism: Structure, Regulation, and Disease Implications.

Advances in experimental medicine and biology·2026
Same journal

The Pyruvate Dehydrogenase Complex: A 90-Year-Old Enigma Shaping the Future of Structural Enzymology.

Advances in experimental medicine and biology·2026
Same journal

Regulation of the Anti-termination RNA Transcription Complex by Lon-Mediated Lambda N Degradation.

Advances in experimental medicine and biology·2026
Same journal

PCNA Macromolecular Complexes: PCNA Serves as a Molecular Hub Regulating Multiple Cellular Processes Inside and Outside of the Nucleus.

Advances in experimental medicine and biology·2026
Same journal

Dynamic Assemblies in Genome Maintenance.

Advances in experimental medicine and biology·2026
See all related articles

High throughput screening identifies inhibitors for plasmepsin II, a malaria parasite target, and cathepsin D. Comparing results reveals enzyme specificity and the utility of encoded combinatorial libraries.

Area of Science:

  • Biochemistry
  • Medicinal Chemistry
  • Parasitology

Background:

  • Combinatorial methods enable synthesis of large compound libraries.
  • High throughput assays are essential for screening these libraries.
  • Aspartyl proteases plasmepsin II and cathepsin D are targets for drug discovery.

Purpose of the Study:

  • To screen compound libraries for inhibitors of plasmepsin II and cathepsin D.
  • To evaluate the specificity of identified inhibitors.
  • To demonstrate the effectiveness of encoded combinatorial libraries.

Main Methods:

  • Utilized high throughput screening assays.
  • Synthesized and screened large compound libraries using combinatorial methods.
  • Compared screening results for plasmepsin II and cathepsin D.

Related Experiment Videos

Main Results:

  • Identified potential inhibitors for both plasmepsin II and cathepsin D.
  • Analysis of results provided insights into enzyme specificity.
  • Demonstrated the power of encoded combinatorial libraries in drug discovery.

Conclusions:

  • High throughput screening of combinatorial libraries is effective for identifying enzyme inhibitors.
  • Comparative analysis of structurally similar enzymes aids in understanding specificity.
  • Plasmepsin II and cathepsin D inhibition are promising therapeutic avenues.