Related Experiment Videos
Sequence-selective RNA scission by oligoamine--DNA conjugates (1)
1Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Japan.
Nucleic Acids Symposium Series
|January 1, 1997
Abstract:
Ethylenediamine and diethylenetriamine, which are active for RNA hydrolysis, are attached to the 5'-ends of synthetic oligonucleotides by urethane linkages. The resultant artificial ribonucleases selectively hydrolyze the substrate RNA at the target phosphodiester linkage. Acid/base cooperation in the oligoamines for the catalysis is confirmed in terms of kinetic evidences.