Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Database searching using mass spectrometry data

J R Yates1

  • 1Department of Molecular Biotechnology, School of Medicine, University of Washington, Seattle 98185-7730, USA. jyates@u.washington.edu

Electrophoresis
|June 25, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Lorentz sphere visualised.

The Journal of chemical physics·2019
Same author

Lonomia obliqua bristle extract modulates Rac1 activation, membrane dynamics and cell adhesion properties.

Toxicon : official journal of the International Society on Toxinology·2019
Same author

Individual differences in impulsive action and dopamine transporter function in rat orbitofrontal cortex.

Neuroscience·2015
Same author

ProLuCID: An improved SEQUEST-like algorithm with enhanced sensitivity and specificity.

Journal of proteomics·2015
Same author

Global quantitative analysis of phosphorylation underlying phencyclidine signaling and sensorimotor gating in the prefrontal cortex.

Molecular psychiatry·2015
Same author

Effects of methylprednisolone and 4-chloro-3-hydroxyanthranilic acid in experimental spinal cord injury in the guinea pig appear to be mediated by different and potentially complementary mechanisms.

Spinal cord·2014
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
Same journal

CMOS Electrokinetic Systems and Fabrication Approaches for On-CMOS 3D Electrodes.

Electrophoresis·2026
See all related articles

Large-scale DNA sequencing and mass spectrometry enable efficient protein identification. These techniques, using peptide mass mapping and tandem mass spectrometry, are vital for analyzing protein biochemistry and identifying proteins in complex mixtures.

Area of Science:

  • Proteomics
  • Bioinformatics
  • Biochemistry

Background:

  • Advancements in DNA sequencing provide a rich sequence infrastructure for protein biochemistry.
  • Mass spectrometry now offers rapid and sensitive determination of peptide weights and amino acid sequences.

Purpose of the Study:

  • To outline methods for protein identification using mass spectrometry and DNA sequencing data.
  • To highlight the utility of peptide mass mapping and tandem mass spectrometry for protein analysis.

Main Methods:

  • Peptide mass mapping: Proteins are digested, and resulting peptide masses are analyzed by mass spectrometry to create a "fingerprint" for identification.
  • Tandem mass spectrometry: Fragmentation patterns and sequence information from tandem mass spectra are used to search sequence and nucleotide databases (e.g., expressed sequence tag sequences).

Related Experiment Videos

Main Results:

  • Peptide mass mapping is effective for protein identification in organisms with completed genomes.
  • Tandem mass spectrometry allows database searching against translated protein and nucleotide sequences, beneficial for incomplete genomes.
  • Tandem mass spectrometry excels at identifying proteins within complex biological mixtures.

Conclusions:

  • Integrated DNA sequencing and mass spectrometry provide powerful tools for protein biochemistry.
  • These methods facilitate protein identification across diverse biological contexts, including complex mixtures and incompletely sequenced genomes.