[Dissociation and culture of primary auditory neurons in postnatal chicken]

In the present study, a primary culture of auditory neurons in 10-16-day old chickens was carried out. We observed the morphologic features of auditory neurons at different culturing stages by using phased-contrast and scanning electron microscope, and particularly described their growth and developmental process. The results indicated that a steady and reliable culturing method established here would contribute to the biological research of inner ear in vitro.