Related Experiment Video

Updated: Aug 5, 2026

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
13:57

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Published on: February 18, 2014

Competitive protein binding assay for biotin monitored by chemiluminescence

H R Schroeder, P O Vogelhut, R J Carrico

    Analytical Chemistry
    |November 1, 1976
    PubMed
    Summary

    No abstract available in PubMed .

    More Related Videos

    In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions08:14

    In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

    Published on: July 17, 2019

    Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes10:43

    Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes

    Published on: October 3, 2019

    Related Concept Videos

    Labeling DNA Probes03:31

    Labeling DNA Probes

    DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
    Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
    Western Blotting01:15

    Western Blotting

    Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
    The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
    Enzyme-Linked Immunosorbent Assay01:33

    Enzyme-Linked Immunosorbent Assay

    In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
    There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.